Enzyme-linked immunosorbent assay or ELISA kits are used for the detection of hundreds of different proteins and molecules present in the given sample. It includes factors responsible for growth, markers used to detect infectious diseases, diabetes, and tumors, etc. These kits are quick, convenient, and accurate for research.
ELISA has different techniques named as sandwich ELISA, competitive ELISA, direct ELISA, and indirect ELISA which is used for the detection of any infection or antibody present in the given sample. These ELISA kits have pre-coated plates which are used to capture and detect any antibody present in the given sample. You can buy high-quality human leptin ELISA kits from https://www.bosterbio.com/human-leptin-picokine-trade-elisa-kit-ek0437-boster.html.
The Human leptin ELISA kit is used to measure the quantity of Hu Leptin in human serum or cell samples. This assay will exclusively identify both natural and combined Hu Leptin in the given sample. It is a type of sandwich ELISA which is designed to quantify the target linked between a matched antibody pair. A target-specific antibody is pre-coated on the wells of the micro-plate.
The sample is then added into these wells and linked to the immobilized antibody. It is formed by the addition of a second antibody which is a substrate solution. It reacts with the complex to produce a signal. To perform a normal ELISA test, you need at least one antibody. The sample with an unknown amount of antigen or antibody is paralyzed on a solid microtiter plate.
After that, the antibody for detection is added into the paralyzed antigen which forms a complex chain with the antigen. Between each step, the plate is washed with a detergent in order to remove any antibodies that come from the atmosphere. In the last, a substrate is added to produce a visible signal, which indicates the quantity of antigen present in the sample.